2P-FCM

* 2P-FCM is a light-weight fiber-coupled head-mounted miniature two-photon microscope (<2 grams) for imaging in awake freely moving mice.
* Employs electrowetting lenses for high-speed variable focusing with no moving parts in a compact design.
* Allows rapid imaging in multiple focal planes and tilted focal planes.

* Uses include imaging neural activity in animals performing complex behavior tasks such as navigating odor plumes or social interactions.

* Mice (Thy1-GCaMP6s, C57BL/6 mouse)

* Prairie voles

* Provides optical sectioned imaging with tunable depth of 200 microns for 3-dimensional imaging.
* Extremely light weight (<2 grams).
* Ability to do fast multiplane imaging and tilted field imaging.

* Lateral resolution is limited to ~ 1.8 microns due to the pixelation from the imaging fiber bundle, although this resolution can still show neural activity from cell soma and processes.

* The 2P-FCM can be added on to any existing two-photon microscope.

Baris N. Ozbay et al. 2018, Three dimensional two-photon brain imaging in freely moving mice using a miniature fiber coupled microscope with active axial-scanning. Scientific Reports 8(1):8108. doi: https://www.nature.com/articles/s41598-018-26326-3 

https://github.com/CUNeurophotonics

https://github.com/CUNeurophotonics

Rocky Mountain Summit on Wearable Miniature Microscopes: https://www.cuneurophotonics.org/conference.html

Patent # US20170010456A1
YouTube video on 2P-FCM: https://youtu.be/rFyFsfgNa3Q